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KMID : 0880220090470030358
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Journal of Microbiology
2009 Volume.47 No. 3 p.358 ~ p.362
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Antibacterial Activity of Recombinant hCAP18/LL37 Protein Secreted from Pichia pastoris
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Kim Soon-Ja
Choi Joong-Kook
Lee Sung-Jin
Lee Hak-Kyo
Quan Renshu
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Abstract
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Human antimicrobial peptide CAP18/LL37 (hCAP18/LL37) was expressed in Pichia pastoris and its antibacterial activity was tested against pathogenic bacteria. The full length ORF of hCAP18/LL37 was cloned into the pPICZαA vector followed by integration into the genomic AOX1 gene of P. pastoris. Agar diffusion assay demonstrated that the different hCAP18/LL37 transformants showed various antibacterial activities against Staphylococcus aureus, Micrococcus luteus, and Salmonella gastroenteritis. The secreted form of hCAP18/LL37 exhibited its maximum activity after 72 h incubation with 2% methanol in MM media, not in BMM. This result suggests that the yeast secreted expression system can be used as a production tool of antimicrobial peptides for industrial or pharmaceutical application.
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KEYWORD
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hCAP18/ LL37, antimicrobial peptide (AMP), antibacterial activity, Pichia pastoris
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